Busch took the white flag as the leader during Coke Zero Sugar 400, but his race came undone as he led the pack down the backstretch.
Harrison Burton flew past Busch to take the lead of the race, aided by a push from Parker Retzlaff, blocking him at the bottom to secure a win that would ensure Burton a spot in the playoffs.
How long will Kyle Busch’s winless streak continue?
Busch’s loss at Daytona has prompted criticism from former NASCAR star Kyle Petty, who has questioned Busch’s ability after the race last weekend.
“Has he forgotten how to speedway race? What in God’s name happened? He has control of the race on a green-white-checkered,” Petty said.
“He has the inside line, [and] he gets there with his friend Christopher Bell who is gonna help him because they were teammates at one point in time.
“They take the green flag, they take control of the race, they run on the bottom of the racetrack that first lap. Harrison Burton and Parker Retzlaff in the No. 62 car, who had the biggest influence on the outcome of the race.